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Virology at IITA

Before joining the MRCG-LSHTM in 2017, I worked on molecular diagnosis of diverse plant-infected viruses from various food crop samples during my internship period at the Virology Molecular Lab of the International Institute of Tropical Agriculture (IITA) Ibadan in 2014/2015 under Dr Lava Kumar. Here I got my first hands-on experience on enzyme immunoassays (ELISA), Nucleic acids extraction, PCR, Agarose gel electrophoresis, recombinant DNA technology, monoclonal antibody production,  protein purification techniques including SDS PAGE, Western Blotting, etc., and their real-life application in plant disease control to enhance the agricultural sector.

Molecular Biology

DNA/RNA extraction PCR & ELISA

Library prep

NGS Seq

Sanger Seq

Primer design

SDS-PAGE

Cell culture

Cell fractionation

Fragment analysis

Gel electrophoresis

Microbiology

Sterilization methods

Optical microscopy

Bacterial staining Plating methods

Cell culture

Use of BSCs Media/buffer prep

Chemistry

HPTLC

Distillation:

-simple

-Fractional

Chromatography:

-Column

-Partition

Other Sep methods

Data Processing

MS Word

Spreadsheets

PowerPoint

Genomic sequence analysis

Python

R

STATA

Linux (Ubuntu)

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