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Mycology & Plant Pathology

At the mycology and pathology laboratories of IITA in 2014, I worked on diagnosing and characterizing fungi Aspergillus Spp from soil and food crops from different countries. This was to support the then-ongoing production of Aspergillus spp (aflatoxin) biocontrol called Aflasafe. Aspergillus culture in microbiology,  Extraction, partition chromatography and HPTLC for species identification were the applied techniques.

Molecular Biology

DNA/RNA extraction PCR & ELISA

Library prep

NGS Seq

Sanger Seq

Primer design

SDS-PAGE

Cell culture

Cell fractionation

Fragment analysis

Gel electrophoresis

Microbiology

Sterilization methods

Optical microscopy

Bacterial staining Plating methods

Cell culture

Use of BSCs Media/buffer prep

Chemistry

HPTLC

Distillation:

-simple

-Fractional

Chromatography:

-Column

-Partition

Other Sep methods

Data Processing

MS Word

Spreadsheets

PowerPoint

Genomic sequence analysis

Python

R

STATA

Linux (Ubuntu)

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